Resources Super Resolution Light

Technical Overviews

Two-photon excitation STED microscopy

Article on obtaining sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy by merging this technique with stimulated-emission depletion (STED).

From: NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
Authors: Gael Moneron and Stefan W. Hell, Opt. Exp. 17 (17), 14567 - 14573
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Light from the dark

Short article on the viewing of Non-fluorescent, light-absorbing molecules using a method that turns them into mini-lasers. Stefan W. Hell and Eva Rittweger, Nature 461, 1069-1070.

From: NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
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Single-molecule biology and Bioimaging Zhuang Research Lab

Zhuang Lab at Harvard University is developing real-time fluorescence imaging methods to track the behavior of single virus particles and of single viral genomes in live cells.

From: , Harvard University
Harvard University
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Fluorescence microscopy with super-resolved optical sections

Alexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005

From: , University of Basel
University of Basel
Authors: Alexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005
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Blinded by the Light

Published in 2004, the article reviews advances in light microscopy including 4Pi, STED, confocal imaging, and total internal reflection fluorescence.

Authors: Bio-IT World Magazine , Rabiya S. Tuma
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Super-Resolution Microscopy Captures Molecules in Motion

A new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.

From: , HHMI Janelia Farm Research Campus
HHMI Janelia Farm Research Campus
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Nonlinear structured-illumination microscopy: Wide-field fluorescence with theoretically unlimited resolution

Article by Mats Gustafsson on saturated structured-illumination microscopy that has a 2D point resolution of less than 50 nm.

Authors: Mats G. L. Gustafsson
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What is Stochastic Optical Reconstruction Microscopy (STORM)

Brief introduction to Stochastic Optical Reconstruction Microscopy (STORM). Includes a reference to peer reviewed articles.

From: , University of California San Francisco
University of California San Francisco
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Breaking the Diffraction Barrier:

Online peer reviewed article that is an overview of the two classes of super-resolution fluorescence microscopes by leading research scientist. Patterned Illumination approach includes stimulated emission depletion (STED) microscopy, RESOLFT technology (Hofmann et al., 2005), and saturated structured illumination microscopy (SSIM). Single molecule imaging that activates individual moelcules at different times includes stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), and fluorescence photoactivation localization microscopy (FPALM)

From: , University of California San Francisco
University of California San Francisco
Authors: Bo Huang, Hazen Babcock, Xiaowei Zhuang
Citation: Cell, Volume 143, Issue 7, 1047-1058, 17 December 2010
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Super resolution microscopy

Well written up to date (as of 2012) article that overviews the types of super resolution microscopes.

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iPALM: resolving the third dimension

From: , HHMI Janelia Farm Research Campus
HHMI Janelia Farm Research Campus
Authors: Lisa Grauer
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Three-dimensional live microscopy beyond the diffraction limit

This 2013 review techniques that are available for three-dimensional, super-resolution live microscopy are discussed. Live cell imaging requires imaging at speeds fast enough to capture dynamic processes without damaging the specimen. Techniques included are Parallelized 4Pi microscopy, 3D structured illumination microscopy, Localization based microscopy, and Reversible saturable optical fluorescence transitions microscopy (RESOLFT).

From: , HHMI Janelia Farm Research Campus
HHMI Janelia Farm Research Campus
Authors: Reto Fiolka
Citation: J. Opt. 15 094002, doi:10.1088/2040-8978/15/9/094002
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Article

Shattering the diffraction limit of light: A revolution in fluorescence microscopy?

Authors: Weiss, S. (2000). Proc. Natl. Acad. Sci. USA 97: 8747-8749.
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STED Microscopy: A New Chapter in Light Imaging

Easy to read article on the invention of the of the STED - stimulation depleted microscopy by Stefan Hell and new directions for the instruments as the basic patent is set to expire in 2015.

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rsEGFP2 enables fast RESOLFT nanoscopy of living cells

RESOLOFT uses lower intensities light than STED that is pulsed in a doughnut so that 100,000 doughnuts can be viewed at one time. This produces a much larger field of view than is available with STED.

From: NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
Authors: Tim Grotjohann, Ilaria Testa, Matthias Reuss, Tanja Brakemann, Christian Eggeling, Stefan W Hell, Stefan Jakobs
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Microscope Development

New Innovations for Biological Imaging and Instrumentation at HHMI

The Applied Physics and Instrumentation Group (APIG) at Janelia Farms seeks to understand bioimaging challenges and to harness new technologies for advanced imaging. Three initial projects will characterize this effort: higher resolution novel microscopy, high-throughput microscopy, and correlative microscopy.

From: , HHMI Janelia Farm Research Campus
HHMI Janelia Farm Research Campus
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The Nobel Prize in Chemistry 2014

"For the development of super-resolved fluorescence microscopy" the Nobel Prize in Chemistry 2014 was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner. The site includes biographic information on the lauretes, advanced background information on super resolution microscopy andpopluar information on how an optical microscope became a nanoscope. Acceptance speeches will also be included on the site.

Citation: The Nobel Prize in Chemistry 2014". Nobelprize.org. Nobel Media AB 2014. Web.
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Lectures and Videos

Intracellular Fluorescent Imaging: Jennifer Lippincott-Schwartz

Three part series on fluorescent imaging and the recent advances in protein markers and instrumentation that allow the visualization and tracking of events with cells at a molecular level. Part 1 is an overview of the principles of fluorescence and the use of GFP has a label for intercellular compartment. Lippincott-Schwartz shows how these labels have been used understand processes in the secretary membrane system. In part two the use of photoactivation and photobleaching (FRAP) to switch on or off specific subsets of fluorescent molecules is described. The three part describes Photo Activated Localization Microscopy (PALM) developed by Eric Betzig and Harald Hess.

From: , NIH National Institute of Child Health and Human Development
NIH National Institute of Child Health and Human Development
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Xiaowei Zhuang Lecture: Super-Resolution Microscopy

A three part iBioEducation lecture series on fluorescence imaging below the diffraction limit of light. Part one is an introduction to Super-Resolution Fluorescence Microscopy with emphases on Stochastic optical reconstruction microscopy (STORM) developed by Zhuang's laboratory. In part two the application of STORM to the study of chormosomes organization of E. coli and to the determination of the molecular structure of a synapse are presented.

From: , Harvard University
Harvard University
Authors: Xiaowei Zhuang
Citation: iBiology
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Stefan Hell on nanometer-scale microscopy in biophotonics

Stefan Hell discusses Stimulated Emission Depletion (STED) microscopy and other far-field optical techniques to noninvasively examine living cells at resolutions better than 20 nm.

From: NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
NanoBiophotonics, Max Plank Institute for Biophysical Chemistry
Authors: Stefan Hell
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Web Portals

Super Resolution Microscopy Leica Science Lab

The Leica site is includes articles by staff, freelance writers, and users, tutorials, and abstracts that are linked to publications. Webinars are available on demand and require registration. The Super Resolution Microscopy contains many resourses on STED, which is part of the Leica product line.

From: Leica Microsystems CMS GmbH
Leica Microsystems CMS GmbH
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Introductions and Briefs

Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structures

This 2012 commentary provides an excellent overview of microscopic techniques used in the 3D imaging of cellular structures. Advances in electron microscopy tomography and fluorescence super-resolution techniques including STED, Single-molecule-localization-based super-resolution, Plane illumination microscopy, and Structured illumination microscopy are reviewed. There is an insightful comparison of super resolution techniques to confocal microscopy in terms of difficulty of preparation, processing and live cell.

From: Super-Resolution Technology Core, University of New Mexico
Super-Resolution Technology Core, University of New Mexico
Authors: Diane S. Lidke and Keith A. Lidke
Citation: June 1, 2012 J Cell Sci 125, 2571-2580., doi: 10.1242/​jcs.090027
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