Resources Two & Multi Photon Microscopy

Techniques

Multiple-photon excitation fluorescence microscopy

An introduction.

From: LOCI Laboratory of Optical and Computational Instrumentation, University of Wisconsin Madison
LOCI Laboratory of Optical and Computational Instrumentation, University of Wisconsin Madison
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Two confocal and SLM Microscopy Publications

Articles on two photon including how to build a two photon and an SLm photon imaging microscope that uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern.

From:

Authors: Rafael Yuste
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Single-molecule Fluorescence Techniques

Recent advancements makes the detection of fluorescence signal from single fluorescent molecule instead of from a huge number of molecules possible.

From: Nature Publishing Group
Nature Publishing Group
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Technical Overviews

Two-photon Fluorescence Light Microscopy

Nice overview article covering the theoretical basis, design of a two-photo microscope, fluorophores, comparisons with conventional and confocal microscopy, photobleaching and photodamage, and application

From: , Massachusetts Institute of Technology
Massachusetts Institute of Technology
Authors: Peter TC So
Citation: Encyclopedia of Life Sciences, 2002
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