Resources Epi Fluorescence

Techniques

Total Internal Reflection Fluorescence Microscopy: Introduction and Applications

From: Nikon Instruments USA
Nikon Instruments USA
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Technical Knowledge for Filters and Fluorescence Microscopy

Knowledge base with an emphasis on selecting filters for fluorescence microscopy applications including TIRF, FRET, Ca2+ ratiometry, Raman, Confocal, Qdots, flow cytometry and all types of fluorescence miccroscopes. Chroma filters are widely used in microscopy.

From: Chroma Technology Corp.
Chroma Technology Corp.
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Green Fluorescent Protein

What is Green Fluorescent Protein and why is it so important.

From: , Connecticut College
Connecticut College
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A blog on fluorescent microscopy in biological research

greenfluorescentblog contains information on fluorescent microscopy techniques as well as current research using advanced fluorescence. This information is up to date and well organized . It is written by Gal Haimovich, a Postdoc at a leading laboratory.

Authors: Gal Haimovich
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Technical Overviews

Concepts in Fluorescence Microscopy

From: Nikon Instruments USA
Nikon Instruments USA
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Fluorescent Proteins

Excellent overview of fluorescent proteins used by biologists as markers of gene expression and protein targeting in intact cells and organisms in order to monitor cellular processes in living systems with fluorescence microscopy.

From: , University of Alberta
University of Alberta
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Nobel Prize 2008 for Green Fluorescent Protein

Osamu Shimomura, Martin Chalfie and Roger Y. Tsien jointly won the Nobel Prize in Chemistry in 2008 for discovery and development of the green fluorescent protein, GFP. Included are the biographies, lectures, interviews, photos, and documentaries on the award.

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Introductions and Briefs

Fluorescence Microscope Basics

A detailed interactive animation that illustrate the basics of imaging in the Fluorescence Microscope.

From: Imaging Technology Group, University of Illinois at Urbana-Champaign
Imaging Technology Group, University of Illinois at Urbana-Champaign
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A New Approach to Fluorescence Microscopy

GE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.

From: , HHMI Janelia Farm Research Campus
HHMI Janelia Farm Research Campus
Authors: Mark Bates
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Multicolor FRET: Adding Colors to the Rainbow

Excellent short article discussing recent advances in FRET including the new fluorescent proteins with distinct colors that can be detected allowing six interactions to me measured at once.

Authors: Sarah C.P. Williams
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Software

The Virtual Microscope

The Virtual Microscope, which is available for free download supports functionality from electron, light, and scanning probe microscopes, datasets for these instruments, training materials to learn more about microscopy, and other related tools.

From: Imaging Technology Group, University of Illinois at Urbana-Champaign
Imaging Technology Group, University of Illinois at Urbana-Champaign
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Open Microscopy Environment

OME (Open Microscopy Environment) develops open-source software and data format standards for the storage and manipulation of biological light microscopy data. A joint project between universities, research establishments and industry in Europe and the USA, OME has over 20 active researchers with strong links to the microscopy community.

From: , University of Dundee
University of Dundee
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Micro-Manager Open Source Microscopy Software

μManager is a software package for control of automated microscopes. Together with the image processing application ImageJ, μManager provides a comprehensive, freely available, imaging solution.

From: , University of California San Francisco
University of California San Francisco
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Investigations & Applications

The mechanism by which chromosomal DNA molecules are held together: entrapment within cohesin rings?

From: , Oxford University
Oxford University
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Cell division drama unfolds under the microscope

From: , Oxford University
Oxford University
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Lectures and Videos

Nico Stuurman (UCSF): Fluorescence Microscopy

Outstanding lecture on fluorescence microscopy covering the theoretical basis, equipment, filter selection, dye types and optimization, and labeling proteins with fluorescent proteins such as green fluorescent protein. The video includes demonstration in the microscope lab.

From: , University of California San Francisco
University of California San Francisco
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Intracellular Fluorescent Imaging: Jennifer Lippincott-Schwartz

Three part series on fluorescent imaging and the recent advances in protein markers and instrumentation that allow the visualization and tracking of events with cells at a molecular level. Part 1 is an overview of the principles of fluorescence and the use of GFP has a label for intercellular compartment. Lippincott-Schwartz shows how these labels have been used understand processes in the secretary membrane system. In part two the use of photoactivation and photobleaching (FRAP) to switch on or off specific subsets of fluorescent molecules is described. The three part describes Photo Activated Localization Microscopy (PALM) developed by Eric Betzig and Harald Hess.

From: , NIH National Institute of Child Health and Human Development
NIH National Institute of Child Health and Human Development
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GFP: Lighting Up Life: Nobel Lecture by Martin Chalfie

After hearing a departmental lunchtime seminar by Paul Brehm that described light production in jelly fish, acqurin and then about a fluorence protein that produced green light instead pf blue, Chalfie became excited about using this protein as a biological marker. The lecture details the events in his laboratory that resulted with with using GFP in C. elegans.

From: , Columbia University
Columbia University
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Constructing and Exploiting the Fluorescent Protein Paintbox: Nobel Lecture Roger Tsien

Roger Tsien changed the emission color and enhanced the fluorescence output of the protein

From: , University of California San Diego
University of California San Diego
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Discovery of Green Fluorescent Protein, GFP: Osamu Shimomura - Nobel Lecture

Lecture detailing Shimomura's research on the biolumininescence of the jellyfish Aequorea that lead to the discovery of Green Fluorescent Protein.

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