Resources Tranmission Electron Microscope

Tutorials in transmission electron microscopy

For biological and material scientists who are new to TEM, but need to learn to use the technique. Includes alignment instructions.

From: Sorby Centre for Electron Microscopy and Microanalysis, University of Sheffield
Sorby Centre for Electron Microscopy and Microanalysis, University of Sheffield
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Course Notes from Dr. Timothy S. Baker

Current course notes for electron microscopy in pdf and PowerPoint. The Bottom Line is especially helpful and focuses basic concepts that are important for understanding the fundamental principles of transmission electron microscopy, biological specimen preparation, and three-dimensional image processing and reconstruction. Winter 2011 course is 3D Electron Microscopy of Macromolecules.

From: Cryoelectron Microscopy Laboratory, University of California San Diego
Cryoelectron Microscopy Laboratory, University of California San Diego
Authors: Timothy S. Baker
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Transmission Electron Microscopy (TEM)

This is a set of resources designed to accompany an introductory course on transmission electron microscopy. The level is appropriate for students with an understanding of some elementary physics.

From: , University of Liverpool
University of Liverpool
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Learn to do TEM

Learn to use a transmission electron microscope...fast. This site has put together by John Rodenburg, who has been teaching graduate students to use TEMs for over twenty years (at the University of Cambridge, Sheffield Hallam and Sheffield Universities). Now includes a new section on TEM/STEM alignment.

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Bioscience Electron Microscopy Course

Highlight of the site is the thirty plus lecture power point presentations developed by John Shields. Topics include specimen preparation for scanning electron and transmission electron microscopy, SEM and TEM instrument design, electron microscopy techniques, and overviews of other types of microscopy. A pdf of the Lab Manual on the preparation of biological specimens for transmission and scanning electron microscopy is available.

From: Georgia Electron Microscopy, University of Georgia
Georgia Electron Microscopy, University of Georgia
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Development of 200kV Atomic Resolution Analytical Microscope

From: JEOL USA, Inc.
JEOL USA, Inc.
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Focused Ion Beam Systems and Dualbeam Systems

A section on focussed ion beam instruments is included in the FEI booklet "An Introduction to Electron Microscopy". The booklet is an excellent overview of of electron microscopy and nanotechnology for students and teachers. It is available as a pdf for download.

From: FEI Company
FEI Company
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Topics in recent studies with high-voltage electron microscopes

High-voltage electron microscopy possesses a number of advantages that cannot be afforded by conventional electron microscopy, thus providing a unique microscopy technique in both materials science and biological science.

Authors: Hirotaro Mori
Citation: J Electron Microsc (Tokyo) (2011) 60 (suppl 1): S189-S197. doi: 10.1093/jmicro/dfr050
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Optimizing Your STEM Microscope

List of publications on optimizing the environment and optimum convergence angles for maximum performance in STEM. All publications are available on the site.

From: , Cornell University
Cornell University
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Disease Carriers: Explore the Microscopic World of Health Science

Transmission electron microscope images of viruses, bacteria, fungi and protozoa disease carriers.

From: , Wadsworth Center
Wadsworth Center
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Intracellular Aspects of the Process of Protein Secretion

George E. Palade – Nobel Lecture, December 12, 1974

From: The Nobel Foundation
The Nobel Foundation
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Shades of gray matter

Summary of research by Xiaokun Sun with Roger Tsien and Mark Ellisman on developing an electron opaque stain that can be genetically tagged to genes of interest and fluoresces. This would allow correlation of the sample from the electron microscope to the light microscope.

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The Electron Microscope as an Illustration of the Wave Nature of the Electron

The transmission electron microscope uses principles that originated in light microscopy and which therefore rely on the properties of waves. These principles are illustrated.

From: , University of Sydney
University of Sydney
Authors: David R McKenzie
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Electron Energy Loss Spectroscopy

Brief introduction to the Principle and application of Imaging electron energy loss spectroscopy

From: Division of Electron Microscopy and Crystal Chemistry, Kyoto University
Division of Electron Microscopy and Crystal Chemistry, Kyoto University
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Dynamic Transmission Electron Microscope

Introduction to the Dynamic Transmission Electron Microscope (DTEM) at Lawrence Livermore Laboratories that provides the ability to image transient behaviors such as how a chemical reaction, structural deformation or phase transformation takes place with an unprecedented combination of spatial and temporal resolution: nanometers and nanoseconds.

From: Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
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Fluctuation Microscopy

Fluctuation microscopy is based on the statistical analysis of the scattering from nanoscopic volumes of the sample. It can be done using dark-field imaging in a conventional TEM. It has been used to study disordered materials including amorphous Ge and silicon and metallic glasses. This paper is a general introduction to the technique.

From: , University of Wisconsin Madison
University of Wisconsin Madison
Authors: Paul Voyles
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Electron Microscopy for Dummies (for Single Particle Reconstruction)

An introduction to the single particle reconstruction electron microscopy and it's applications to biology. The site includes a brief introduction to the transmission electron microscope, and more detailed information on preparation of biological sections using grids, staining and freezing and Single Particle Reconstruction. Last update was Nov 2012.

Authors: Hanspeter Niederstrasser
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Movie Mode Dynamic Transmission Electron Microscopy (DTEM)

The Dynamic Transmission Electron Microscope (DTEM) at Lawrence Livermore National Laboratory has the capability of capturing micro- and nano-structural events in multiframe movies. Applications include direct viewing at the microscale of material structural changes and chemical reactions.

From: Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
Authors: Rosae Hansen
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The Nobel Prize in Physics 1986

The Royal Swedish Academy of Sciences has decided to award the 1986 Nobel Prize in Physics by one half to Professor. Ernst Ruska, Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, Federal Republic of Germany, for his fundamental work in electron optics, and for the design of the first electron microscope and the other half, jointly to Dr Gerd Binnig and Dr Heinrich Rohrer, IBM Research Laboratory, Zurich, Switzerland, for their design of the scanning tunnelling microscope.

From: The Nobel Foundation
The Nobel Foundation
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The Development of the Electron Microscope and of Electron Microscopy

From Nobel Lectures, Physics 1981-1990, Editor-in-Charge Tore Frängsmyr, Editor Gösta Ekspång, World Scientific Publishing Co., Singapore, 1993

From: The Nobel Foundation
The Nobel Foundation
Authors: Ernst Ruska
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Journey Into the Cell

History of electron microscopy at Rockefeller University and the

From: Rockefeller News
Rockefeller News
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From Intracellular Morphology to Function: The Secretory Pathway

Working in Albert Claude research group at Rockefeller University, George Palade developed the sucrose method for cell fractionation which isolated organelles without distorting their shapes. By using osmium tetroxide better contrast was obtained in electron microscopy and Palade was able to describe the fine structure of mitochondria. This summary of Palade's work at Rockefeller University include a link references to journal articles.

From: Bio-Imaging Resource Center, Rockefeller University
Bio-Imaging Resource Center, Rockefeller University
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Cell Fractionation, Biochemistry, and Electron Microscopy: The Birth of Modern Cell Biology

Albert Claude pioneered techniques of cell fractionation and was the first to use an electron microscopes to study cells. This first image of an intact cell, a collaboration with Keith Portor and Ernest Fullam, was termed it the "birth certificate" of the field of cell biology by George Palade. This summary of his work at Rockefeller University includes links to selected publications.

From: Bio-Imaging Resource Center, Rockefeller University
Bio-Imaging Resource Center, Rockefeller University
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Eva Nogales: Introduction to Electron Microscopy

The lecture covers transmission electron microscope image formation, interaction of electrons with samples, biological specimen preparation for TEM, the types of TEM instruments, and capabilities for the examination of biological specimens using from work in the Nogales lab. Image processing methods for obtaining three-dimensional are presented.

From: , University of California Berkeley
University of California Berkeley
Authors: Eva Nogales
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