Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structures

Diane S. Lidke and Keith A. Lidke, Super-Resolution Technology Core, University of New Mexico
Journal of Cell Biology

This 2012 commentary provides an excellent overview of microscopic techniques used in the 3D imaging of cellular structures. Advances in electron microscopy tomography and fluorescence super-resolution techniques including STED, Single-molecule-localization-based super-resolution, Plane illumination microscopy, and Structured illumination microscopy are reviewed. There is an insightful comparison of super resolution techniques to confocal microscopy in terms of difficulty of preparation, processing and live cell.