Three-dimensional electron crystallography of protein microcrystals

Dan Shi, Brent L Nannenga, Matthew GIadanza, Tamir Gonen, HHMI Janelia Farm Research Campus

Using a new protocal electron diffraction data from protein crystals was collected at 2.9 Å resolution using electron diffraction in a cryo electron microscope and result in. The protocol used an electron diffraction collected at resolutions of 1.7 Å. A full tilt series of 90 diffraction patterns was collected from three crystals. The technique is named ‘MicroED’.