Buyers Guide: Cryo Preparation Systems including SEM

Rapid Immersion Plunge Freezer

Electron Microscopy Sciences Web Link

The EMS-002 achieves ultra-rapid cooling by quenching the sample in liquid propane or other cryogen, cooled to near liquid nitrogen temp by using a stainless steel vacuum insulated Dewar and copper condensation chamber.

Freeze Substitution Kit

Electron Microscopy Sciences Web Link

EMS Freeze substitution kit enables low temperature dehydration and fixation of rapidly frozen cells of a small volume of cell in as little as 90 minutes instead of days. Cells such as cuticles or thick cell wall that have significant diffusion barriers or a larger volume can extend the time to 3 hours. The kit is based on the work of Webb & McDonald, (2011) Freeze Substitution in 3 hours or less. J Microscopy 243, 227-233.

Freeze Driers for SEM and TEM Preparation

Electron Microscopy Sciences Web Link

The EMS 775 Turbo Freeze Dryer operates at temperatures down to -140°C and employs a Turbomolecular pumping unit, backed by a Rotary Vacuum pump. The lower temperatures are achieved by using a Liquid Nitrogen fed Cold Stage.